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OSMOSIS 5, SUMMER 1993

 

Contents

Doubling and dabbling in duckweed
A simple way to study the behaviour of the hypocotyls (stalks) of small seedlings
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Doubling and dabbling in duckweed

Duckweed does not immediately present an exciting image to the young scientist. It is just green, has no flowers (that you can see), it is small and just sits there on the pond. But have you ever looked closely at your local duckweeds? There are at least 5 different species in Britain. The one you will know best is, no doubt, the lesser duckweed, Lemna minor, and this is certainly worth plenty of attention as an ideal plant for investigations. But before we sing its praises as an experimental organism lets introduce the rest of the family!

The duckweeds (Family Lemnacea) are a small and cosmopolitan group, found from the sub-polar regions to the tropics. They all prefer ditches, ponds, lakes and slow-flowing rivers. There are, surprisingly, only 22 species in the world and most are very widespread. One can imagine them jet-setting from one continent to another on the feet of birds. All have a few flattened, often rounded, disc-like leaves (they are really leaf-like stems or thalli) floating on the surface. New leaves bud off from these as the plant grows. Once there are half a dozen leaves the plants break apart. Often there is an unbranched rootlet hanging below the leaves. Some of these rootlets are photosynthetic. The table and diagrams describe some duckweeds you may find in your locality, identified by their individual leaf size, leaf shape and rootlet characteristics.

Lemna minor is undoubtedly the most often encountered, but the smaller Lemna minuta has recently been spreading rapidly in Britain. Spirodela polyrhiza is less common but much the largest and most elegant.

Experiments with duckweeds.

Investigating optimal growth conditions.

  • Surprisingly fast rates of growth are achieved if plants are grown next to bright strip lights at 25 C. Optimal rates are with more than 15 hours of light per day. They need rich nutrients, plants grow well with about 50 grammes of potting compost to the litre of water. Experiments can be conducted on the nutrient requirements, on the effectiveness of different types of artificial light, its intensity, colour (wavelength) and on total day length. Colour filters and an electric timer are useful for such projects. Duckweed can be grown easily in a variety of containers such as old yoghurt pots.

Studying growth and measuring its rate.

  • Daily counts of the numbers of leaves will give a rapid estimate of growth. Graphed, these will show an exponential (multiplicatory) rate. On a log plot there should be a straight line, until factors become limiting. How fast a growth can you achieve and how short a doubling time? Can you work out a mathematical formula for calculating duckweed doubling time? If you cannot, challenge your Maths department! The fastest duckweed I have recorded has a doubling time of 2.4 days. Can you beat it? Once an alien duckweed invaded a lake in Africa and covered 200 square kilometres in 6 months...duckweeds can be fast plants.
  • A fraction of the duckweed can be collected every few days from the culture and dried on a filter paper to estimate dry weight productivity (use the top of your light-bank as a drier). You need a fine balance. Duckweed weight gained, per unit area, approaches yields comparable to cereals! Duckweeds are readily eaten by ducks. In the summer the ducks certainly cannot keep pace with duckweed production. What area of duck-pond does a duckweed-feeding duck need? Do goldfish eat duckweed?

     

    Species

    Leaf size

    Leaf character

    Rootlets

    The great duckweed Spirodela polyrhiza

    large 5 - 8 mm

    shiny and circular, floating

    many up to 30 mm long

    The lesser duckweed Lemna minor

    small 1.5 - 4 mm

    opaque and more or less elliptical, floating

    single to 15 mm long

    The least duckweed Lemna minuta

    very small 1 - 2.5 mm

    elliptical, floating

    single to 8 mm

    The ivy-leaf duckweed Lemna trisulca

    elongated 5 to 15 mm

    spear-shaped with opposite branches, submerged and translucent

    single and hooked

    The gibbous duckweed Lemna gibba

    very small 3 - 5 mm

    ovate, convex below, fat and bouyant

    single to 6 mm

Competition between species

Try growth rate races between different species.

  • Do they compete best under the same or under different conditions?
  • If you can get 2 different species to grow well, you could let them fight it out in a beaker.
  • Which species does best in the end?
  • Why did the winner win? What is the adaptive significance in the diversity of root shapes and lengths?
  • Why are some duckweeds rare?

Report back to SAPS on your success with doubling and dabbling! In the next Osmosis, we shall supply the duckweed doubling formula and deal with duckweeds and pollution... a wonderful subject for more experiments.

Stephen Tomkins

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Noticeboard - Your questions answered

Q. Most science journals seem to be aimed at teachers. Are there any which are written for students?

  • Yes, there are at least two. Catalyst which is aimed at students doing GCSE science and separate sciences, and Biological Sciences Review which is written for Post-16 students. Both journals are excellent and are published by Philip Allen, Market Place, Deddington, Oxon OX15 0SE, UK. Tel: 01869 38652. Reduced rate student subscriptions are available.

Q. There seems to be a lot of information and advice on growing plants available from lots of different sources. Has anyone gathered all this information into one convenient package?

  • Yes! The CLEAPSS Laboratory Handbook has an excellent section devoted to plants. This contains information about a selection of useful plants including how to grow them, where to get them, what topics they are useful for, etc. The Handbook is available (in the UK) from: CLEAPSS School Science Service, Brunel University, Uxbridge UB8 3PH, UK. Tel: 01895 251496.

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